Polyclonal IgM and IgA block in vitro complement deposition mediated by anti-ganglioside antibodies in autoimmune neuropathies.

Sudo, Makoto; Miyaji, Kazuki; Späth, Peter Julius; Morita-Matsumoto, Kana; Yamaguchi, Yoshiki; Yuki, Nobuhiro (2016). Polyclonal IgM and IgA block in vitro complement deposition mediated by anti-ganglioside antibodies in autoimmune neuropathies. International immunopharmacology, 40, pp. 11-15. Elsevier 10.1016/j.intimp.2016.08.019

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Intravenous immunoglobulin (IVIG), consisting of IgG, is the first-line treatment for Guillain-Barré syndrome and multifocal motor neuropathy. IgG, but neither IgM nor IgA, has been demonstrated in vitro to inhibit complement deposition mediated by anti-ganglioside autoantibodies in sera from patients with both conditions. The objective of this study is to investigate the in vitro effectiveness of IgM and IgA in inhibiting complement deposition to ganglioside/anti-ganglioside antibody complexes. Serum samples were obtained from patients with multifocal motor neuropathy associated with anti-GM1 IgM antibodies, Guillain-Barré syndrome associated with anti-GM1 IgG antibodies and Miller Fisher syndrome associated with anti-GQ1b IgG antibodies. Inhibition of complement deposition using different immunoglobulin preparations was measured by enzyme-linked immunosorbent assay. IgM/A-enriched IVIG and immunoglobulin isotypes (polyclonal IgM and IgA) showed higher potential in inhibiting complement deposition than standard IVIG. Although the safety concerns about the use of IgM and IgA for an immunotherapy still remain, IgM and IgA may serve as an alternative immunotherapy in those anti-ganglioside antibody-mediated neuropathies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

Späth, Peter Julius

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1567-5769

Publisher:

Elsevier

Language:

English

Submitter:

Jana Berger

Date Deposited:

20 Mar 2018 16:54

Last Modified:

20 Mar 2018 16:54

Publisher DOI:

10.1016/j.intimp.2016.08.019

PubMed ID:

27567246

Uncontrolled Keywords:

Anti-ganglioside antibody Complement Guillain–Barré syndrome Intravenous immunoglobulin Multifocal motor neuropathy

BORIS DOI:

10.7892/boris.101400

URI:

https://boris.unibe.ch/id/eprint/101400

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