Towards Unidirectional Reconstitution Of Membrane Proteins Into Liposomes

Amati, Andrea Marco; von Ballmoos, Christoph (25 September 2016). Towards Unidirectional Reconstitution Of Membrane Proteins Into Liposomes (Unpublished). In: Annual Meeting of the German Biophysical Society 2016. Erlangen, Deutschland. 25.-28. September 2016.

Functional studies of membrane proteins (MP) in their native environment (the cellular membrane) is challenging due to the complexity of the native membranes and measurement interferences from other membrane constituents or reactions. Therefore, MPs are often purified and integrated into systems which mimic their natural environment in a membrane as e.g. liposomes or nanodiscs, a process called reconstitution, allowing to investigate its function and structural aspects without any disturbing background. The most important problem during MP-reconstitution is the often random orientation of the MP in the liposomal membrane after reconstitution. For functional studies of the MP of interest and quantitative analysis of its properties, unidirectional orientation in the liposomal membrane is required. Previous work of other groups, where MPs have been reconstituted and studied, did not include a final and universal approach (Rigaud JL, Levy D. (2003) Methods Enzymol. 372:65-86.; Tunuguntla R et al. (2013) Biophys. J. 105(6):1388-96.) and procedures have to be individually optimized for an enrichment of enzyme orientation. In most cases, however, orientation cannot be influenced and is thought to depend on the three-dimensional structure of the protein (Tunuguntla R et al. (2013) Biophys. J. 105(6):1388-96; Nordlund G et al. (2014) Nat. Commun. 5:4303.). We are currently developing and establishing a universal method to force unidirectional reconstitution of MPs by the aid of a molecular unit that can be attached to every protein. Our method is based on the observation that the F1F0 ATP synthase with its large hydrophilic head piece (F1 part) orients highly uniformly when embedded in preformed vesicles. We try to mimic this situation with a bioengineering approach that should be applicable to a wide variety of proteins. Once such a method is fully established, the interplay of two or more membrane proteins can be investigated more quantitatively.

Item Type:

Conference or Workshop Item (Poster)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Amati, Andrea Marco and von Ballmoos, Christoph

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

Language:

English

Submitter:

Andrea Marco Amati

Date Deposited:

27 Jul 2017 08:41

Last Modified:

27 Jul 2017 08:41

URI:

https://boris.unibe.ch/id/eprint/101411

Actions (login required)

Edit item Edit item
Provide Feedback