Cbp80 is needed for the expression of piRNA components and piRNAs

Rashpa, Ravish; Vazquez Pianzola, Maria Paula; Colombo, Martino; Hernandez, Greco; Beuchle, Dirk; Berger, Fabienne Chantal; Peischl, Stephan; Bruggmann, Rémy; Suter, Beat (2017). Cbp80 is needed for the expression of piRNA components and piRNAs. PLoS ONE, 12(7), e0181743. Public Library of Science 10.1371/journal.pone.0181743

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Cap binding protein 80 (Cbp80) is the larger subunit of the nuclear cap-binding complex (nCBC), which is known to play important roles in nuclear mRNA processing, export, stabil- ity and quality control events. Reducing Cbp80 mRNA levels in the female germline revealed that Cbp80 is also involved in defending the germline against transposable elements. Com- bining such knockdown experiments with large scale sequencing of small RNAs further showed that Cbp80 is involved in the initial biogenesis of piRNAs as well as in the secondary biogenesis pathway, the ping-pong amplification cycle. We further found that Cbp80 knock- down not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway. Furthermore, compared to con- trols, levels of Piwi and Aub were also reduced upon knock down of Cbp80. On the other hand, with the same treatment we could not detect significant changes in levels or subcellu- lar distribution (nuage localization) of piRNA precursor transcripts. This shows that Cbp80 plays an important role in the production and localization of the protein components of the piRNA pathway and it seems to be less important for the production and export of the piRNA precursor transcripts.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > RNA
08 Faculty of Science > Departement of Chemistry and Biochemistry
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Drosophila
08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Bioinformatics

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Rashpa, Ravish; Vazquez Pianzola, Maria Paula; Colombo, Martino; Beuchle, Dirk; Berger, Fabienne Chantal; Peischl, Stephan; Bruggmann, Rémy and Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
500 Science

ISSN:

1932-6203

Publisher:

Public Library of Science

Funders:

[42] Schweizerischer Nationalfonds
[UNSPECIFIED] Canton of Bern / University of Bern
[UNSPECIFIED] Equal opportunity grant form the Phil.-nat. Fakulta ̈t to P.V.-P.
[UNSPECIFIED] NOMIS Foundation (nomisfoundation.ch) grant to Marc-David Ruepp

Language:

English

Submitter:

Beat Suter

Date Deposited:

18 Aug 2017 16:05

Last Modified:

18 Aug 2017 16:05

Publisher DOI:

10.1371/journal.pone.0181743

PubMed ID:

28746365

BORIS DOI:

10.7892/boris.102492

URI:

https://boris.unibe.ch/id/eprint/102492

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