Imaging samples larger than the field of view: the SLS experience

Vogiatzis Oikonomidis, Ioannis; Lovric, Goran; Cremona, Tiziana Patrizia; Arcadu, Filippo; Patera, Alessandra; Schittny, Johannes; Stampanoni, Marco (2017). Imaging samples larger than the field of view: the SLS experience. Journal of Physics: Conference Series, 849, 012004. Institute of Physics Publishing IOP 10.1088/1742-6596/849/1/012004

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Volumetric datasets with micrometer spatial and sub-second temporal resolutions are nowadays routinely acquired using synchrotron X-ray tomographic microscopy (SRXTM). Although SRXTM technology allows the examination of multiple samples with short scan times, many specimens are larger than the field-of-view (FOV) provided by the detector. The extension of the FOV in the direction perpendicular to the rotation axis remains non-trivial. We present a method that can efficiently increase the FOV merging volumetric datasets obtained by region-ofinterest tomographies in di↵erent 3D positions of the sample with a minimal amount of artifacts and with the ability to handle large amount of data. The method has been successfully applied for the three-dimensional imaging of a small number of mouse lung acini of intact animals, where pixel sizes down to a micrometer range, as well as short exposure times are required.

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