Imaging samples larger than the field of view: the SLS experience

Vogiatzis Oikonomidis, Ioannis; Lovric, Goran; Cremona, Tiziana Patrizia; Arcadu, Filippo; Patera, Alessandra; Schittny, Johannes; Stampanoni, Marco (2017). Imaging samples larger than the field of view: the SLS experience. Journal of Physics: Conference Series, 849, 012004. Institute of Physics Publishing IOP 10.1088/1742-6596/849/1/012004

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Volumetric datasets with micrometer spatial and sub-second temporal resolutions are nowadays routinely acquired using synchrotron X-ray tomographic microscopy (SRXTM). Although SRXTM technology allows the examination of multiple samples with short scan times, many specimens are larger than the field-of-view (FOV) provided by the detector. The extension of the FOV in the direction perpendicular to the rotation axis remains non-trivial. We present a method that can efficiently increase the FOV merging volumetric datasets obtained by region-ofinterest tomographies in di↵erent 3D positions of the sample with a minimal amount of artifacts and with the ability to handle large amount of data. The method has been successfully applied for the three-dimensional imaging of a small number of mouse lung acini of intact animals, where pixel sizes down to a micrometer range, as well as short exposure times are required.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Vogiatzis Oikonomidis, Ioannis; Cremona, Tiziana Patrizia and Schittny, Johannes

Subjects:

500 Science > 530 Physics
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1742-6588

Publisher:

Institute of Physics Publishing IOP

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

13 Oct 2017 16:30

Last Modified:

15 Oct 2017 02:24

Publisher DOI:

10.1088/1742-6596/849/1/012004

BORIS DOI:

10.7892/boris.105154

URI:

https://boris.unibe.ch/id/eprint/105154

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