Recent advances in the development and use of molecular tests to predict antimicrobial resistance in Neisseria gonorrhoeae.

Donà, Valentina; Low, Nicola; Golparian, Daniel; Unemo, Magnus (2017). Recent advances in the development and use of molecular tests to predict antimicrobial resistance in Neisseria gonorrhoeae. Expert review of molecular diagnostics, 17(9), pp. 845-859. Expert Reviews 10.1080/14737159.2017.1360137

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INTRODUCTION The number of genetic tests, mostly real-time PCRs, to detect antimicrobial resistance (AMR) determinants and predict AMR in Neisseria gonorrhoeae is increasing. Several of these assays are promising, but there are important shortcomings and few assays have been adequately validated and quality assured. Areas covered: Recent advances, focusing on publications since 2012, in the development and use of molecular tests to predict gonococcal AMR for surveillance and for clinical use, advantages and disadvantages of these tests and of molecular AMR prediction compared with phenotypic AMR testing, and future perspectives for effective use of molecular AMR tests for different purposes. Expert commentary: Several challenges for direct testing of clinical, especially extra-genital, specimens remain. The choice of molecular assay needs to consider the assay target, quality controls, sample types, limitations intrinsic to molecular technologies, and specific to the chosen methodology, and the intended use of the test. Improved molecular- and particularly genome-sequencing-based methods will supplement AMR testing for surveillance purposes, and translate into point-of-care tests that will lead to personalized treatments, while sparing the last available empiric treatment option (ceftriaxone). However, genetic AMR prediction will never completely replace phenotypic AMR testing, which detects also AMR due to unknown AMR determinants.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Donà, Valentina and Low, Nicola

Subjects:

600 Technology > 610 Medicine & health
300 Social sciences, sociology & anthropology > 360 Social problems & social services
500 Science > 570 Life sciences; biology

ISSN:

1473-7159

Publisher:

Expert Reviews

Language:

English

Submitter:

Tanya Karrer

Date Deposited:

17 Oct 2017 09:54

Last Modified:

04 Aug 2018 02:30

Publisher DOI:

10.1080/14737159.2017.1360137

PubMed ID:

28741392

Uncontrolled Keywords:

Neisseria gonorrhoeae, antimicrobial resistance, molecular testing, NAAT, ceftriaxone, ciprofloxacin, azithromycin, penA, gyrA, 23S rRNA

BORIS DOI:

10.7892/boris.105754

URI:

https://boris.unibe.ch/id/eprint/105754

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