Virosome-bound antigen enhances DC-dependent specific CD4(+) T cell stimulation, inducing a Th1 and Treg profile in vitro.

Blom, Rebecca Agaatha Maria; Amacker, Mario; Moser, Christian; van Dijk, R Maarten; Bonetti, Raffaela; Seydoux, Emilie; Hall, Sean; von Garnier, Christophe; Blank, Fabian (2017). Virosome-bound antigen enhances DC-dependent specific CD4(+) T cell stimulation, inducing a Th1 and Treg profile in vitro. Nanomedicine : nanotechnology, biology, and medicine, 13(5), pp. 1725-1737. Elsevier 10.1016/j.nano.2017.02.004

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There is considerable interest to develop antigen-carriers for immune-modulatory clinical applications, but insufficient information is available on their effects on antigen-presenting cells. We employed virosomes coupled to ovalbumin (OVA) to study their interaction with murine bone marrow-derived dendritic cells (BMDCs) and modulation of downstream T cell responses. BMDCs were treated in vitro with virosomes or liposomes prior to determining BMDC phenotype, viability, and intracellular trafficking. Antigen-specific CD4(+) T cell activation was measured by co-culture of BMDCs with DO11.10 CD4(+) T cells. Compared to liposomes, virosomes were rapidly taken up. Neither nanocarrier type affected BMDC viability, nor did a moderate degree of activation differ for markers such as CD40, CD80, CD86. Virosome uptake occurred via clathrin-mediated endocytosis and phagocytosis, with co-localization in late endosomes. Only BMDCs treated with OVA-coupled virosomes induced enhanced OVA-specific CD4(+) T cell proliferation. Antigen-coupled virosomes are endowed with an intrinsic ability to modulate DC-dependent adaptive immune responses.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Thoraxchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
09 Interdisciplinary Units > Microscopy Imaging Center MIC
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Live Cell Imaging (LCI)

UniBE Contributor:

Blom, Rebecca Agaatha Maria; Amacker, Mario; Hall, Sean; von Garnier, Christophe and Blank, Fabian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1549-9642

Publisher:

Elsevier

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

27 Nov 2017 10:45

Last Modified:

19 Feb 2019 14:38

Publisher DOI:

10.1016/j.nano.2017.02.004

PubMed ID:

28214610

Uncontrolled Keywords:

CD4(+) T cells Immune modulation Liposomes Mouse dendritic cells Virosomes

BORIS DOI:

10.7892/boris.106606

URI:

https://boris.unibe.ch/id/eprint/106606

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