Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei.

Agop Nersesian, Carolina; De Niz Hidalgo, Mariana Isabel; Niklaus, Livia; Prado, Monica; Eickel, Nina; Heussler, Volker (2017). Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei. Scientific Reports, 7(1), p. 2191. Nature Publishing Group 10.1038/s41598-017-02156-7

[img]
Preview
Text
Agop-Nersesian_Sci Report_2017.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (3MB) | Preview

The hepatic stage of the malaria parasite Plasmodium is accompanied by an autophagy-mediated host response directly targeting the parasitophorous vacuolar membrane (PVM) harbouring the parasite. Removal of the PVM-associated autophagic proteins such as ubiquitin, p62, and LC3 correlates with parasite survival. Yet, it is unclear how Plasmodium avoids the deleterious effects of selective autophagy. Here we show that parasites trap host autophagic factors in the tubovesicular network (TVN), an expansion of the PVM into the host cytoplasm. In proliferating parasites, PVM-associated LC3 becomes immediately redirected into the TVN, where it accumulates distally from the parasite's replicative centre. Finally, the host factors are shed as vesicles into the host cytoplasm. This strategy may enable the parasite to balance the benefits of the enhanced host catabolic activity with the risk of being eliminated by the cell's cytosolic immune defence.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Agop Nersesian, Carolina; De Niz Hidalgo, Mariana Isabel; Niklaus, Livia; Eickel, Nina and Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Funders:

[42] Schweizerischer Nationalfonds
[UNSPECIFIED] SystemsX

Language:

English

Submitter:

Volker Heussler

Date Deposited:

01 Dec 2017 11:05

Last Modified:

16 Jan 2019 15:24

Publisher DOI:

10.1038/s41598-017-02156-7

PubMed ID:

28526861

BORIS DOI:

10.7892/boris.107201

URI:

https://boris.unibe.ch/id/eprint/107201

Actions (login required)

Edit item Edit item
Provide Feedback