A Plasmodium plasma membrane reporter reveals membrane dynamics by live-cell microscopy.

Burda, Paul-Christian; Schaffner, Marco; Kaiser, Gesine; Roques, Magali Hélène Yvonne; Zuber, Benoît; Heussler, Volker (2017). A Plasmodium plasma membrane reporter reveals membrane dynamics by live-cell microscopy. Scientific Reports, 7(1), p. 9740. Nature Publishing Group 10.1038/s41598-017-09569-4

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During asexual replication within the Anopheles mosquito and their vertebrate host, Plasmodium parasites depend on the generation of a massive amount of new plasma membrane to produce thousands of daughter parasites. How the parasite plasma membrane (PPM) is formed has mostly been studied by electron microscopy, which does not allow an insight into the dynamics of this process. We generated a Plasmodium berghei reporter parasite line by GFP-tagging of a non-essential PPM-localized protein, and followed plasma membrane development in living parasites through the entire Plasmodium life cycle. By generating double-fluorescent parasites in which the PPM is visualized in combination with the parasite endoplasmic reticulum, we show that membrane contact sites are formed between both membrane systems during oocyst and liver stage development that might be used to deliver lipids to the dramatically expanding PPM. In conclusion, we have established a powerful tool to follow PPM development in living parasites, which promises to greatly expand our knowledge of membrane biology in the Plasmodium parasite.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Burda, Paul-Christian; Kaiser, Gesine; Roques, Magali Hélène Yvonne; Zuber, Benoît and Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Funders:

[42] Schweizerischer Nationalfonds
[UNSPECIFIED] SystemsX

Language:

English

Submitter:

Volker Heussler

Date Deposited:

01 Dec 2017 11:16

Last Modified:

23 Oct 2019 00:24

Publisher DOI:

10.1038/s41598-017-09569-4

PubMed ID:

28851956

BORIS DOI:

10.7892/boris.107203

URI:

https://boris.unibe.ch/id/eprint/107203

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