Questioning the role of selected somatic PIK3C2B mutations in squamous non-small cell lung cancer oncogenesis.

Kind, Marcus Johann; Klukowska-Rötzler, Jolanta; Berezowska, Sabina Anna; Arcaro, Alexandre; Charles, Roch-Philippe (2017). Questioning the role of selected somatic PIK3C2B mutations in squamous non-small cell lung cancer oncogenesis. PLoS ONE, 12(10), e0187308. Public Library of Science 10.1371/journal.pone.0187308

[img]
Preview
Text
file.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (5MB) | Preview

PI3K signaling is frequently dysregulated in NSCLC-SQCC. In contrast to well characterized components of the PI3K signaling network contributing to the formation of SQCC, potential oncogenic effects of alterations in PIK3C2B are poorly understood. Here, a large cohort (n = 362) of NSCLC-SQCC was selectively screened for four reported somatic mutations in PIK3C2B via Sanger sequencing. In addition, two mutations leading to an amino acid exchange in the kinase domain (C1181, H1208R) were examined on a functional level. None of the mutations were identified in the cohort while well characterized hotspot PIK3CA mutations were observed at the expected frequency. Ultimately, kinase domain mutations in PI3KC2β were found to have no altering effect on downstream signaling. A set of SQCC tumors sequenced by The Cancer Genome Atlas (TCGA) equally indicates a lack of oncogenic potential of the kinase domain mutations or PIK3C2B in general. Taken together, this study suggests that PIK3C2B might only have a minor role in SQCC oncogenesis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > University Emergency Center
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hämatologie / Onkologie (Pädiatrie)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hämatologie / Onkologie (Pädiatrie)

UniBE Contributor:

Kind, Marcus Johann; Klukowska-Rötzler, Jolanta; Berezowska, Sabina Anna; Arcaro, Alexandre and Charles, Roch-Philippe

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Sabina Anna Berezowska

Date Deposited:

23 Nov 2017 10:01

Last Modified:

06 Feb 2018 14:33

Publisher DOI:

10.1371/journal.pone.0187308

PubMed ID:

29088297

BORIS DOI:

10.7892/boris.107267

URI:

https://boris.unibe.ch/id/eprint/107267

Actions (login required)

Edit item Edit item
Provide Feedback