Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish.

Conedera, Federica Maria; Arendt, Petra; Trepp, Carolyn Mary; Tschopp, Markus; Enzmann, Volker (2017). Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish. Journal of visualized experiments(128) MYJoVE Corporation 10.3791/56249

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A fascinating difference between teleost and mammals is the lifelong potential of the teleost retina for retinal neurogenesis and regeneration after severe damage. Investigating the regeneration pathways in zebrafish might bring new insights to develop innovative strategies for the treatment of retinal degenerative diseases in mammals. Herein, we focused on the induction of a focal lesion to the outer retina in adult zebrafish by means of a 532 nm diode laser. A localized injury allows investigating biological processes that take place during retinal degeneration and regeneration directly at the area of damage. Using non-invasive optical coherence tomography (OCT), we were able to define the location of the damaged area and monitor subsequent regeneration in vivo. Indeed, OCT imaging produces high-resolution, cross-sectional images of the zebrafish retina, providing information which was previously only available with histological analyses. In order to confirm the data from real-time OCT, histological sections were performed and regenerative response after the induction of the retinal injury was investigated by immunohistochemistry.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Conedera, Federica Maria; Arendt, Petra; Trepp, Carolyn Mary; Tschopp, Markus and Enzmann, Volker

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-087X

Publisher:

MYJoVE Corporation

Language:

English

Submitter:

Volker Enzmann

Date Deposited:

27 Feb 2018 11:01

Last Modified:

22 Oct 2019 18:40

Publisher DOI:

10.3791/56249

PubMed ID:

29155720

BORIS DOI:

10.7892/boris.108511

URI:

https://boris.unibe.ch/id/eprint/108511

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