Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei

Käser, Sandro; Willemin, Mathilde Stéphanie; Schnarwiler, Felix; Schimanski, Bernd; Poveda-Huertes, Daniel; Oeljeklaus, Silke; Haenni, Beat; Zuber, Benoît; Warscheid, Bettina; Meisinger, Christof; Schneider, André (2017). Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei. PLoS pathogens, 13(12), e1006808. Public Library of Science 10.1371/journal.ppat.1006808

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Mitochondria cannot form de novo but require mechanisms that mediate their inheritance to daughter cells. The parasitic protozoan Trypanosoma brucei has a single mitochondrion with a single-unit genome that is physically connected across the two mitochondrial membranes with the basal body of the flagellum. This connection, termed the tripartite attachment complex (TAC), is essential for the segregation of the replicated mitochondrial genomes prior to cytokinesis. Here we identify a protein complex consisting of three integral mitochondrial outer membrane proteins-TAC60, TAC42 and TAC40-which are essential subunits of the TAC. TAC60 contains separable mitochondrial import and TAC-sorting signals and its biogenesis depends on the main outer membrane protein translocase. TAC40 is a member of the mitochondrial porin family, whereas TAC42 represents a novel class of mitochondrial outer membrane β-barrel proteins. Consequently TAC40 and TAC42 contain C-terminal β-signals. Thus in trypanosomes the highly conserved β-barrel protein assembly machinery plays a major role in the biogenesis of its unique mitochondrial genome segregation system.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Departement of Chemistry and Biochemistry
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Käser, Sandro; Willemin, Mathilde Stéphanie; Schnarwiler, Felix; Schimanski, Bernd; Haenni, Beat; Zuber, Benoît; Meisinger, Christof and Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
600 Technology > 610 Medicine & health

ISSN:

1553-7366

Publisher:

Public Library of Science

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

11 Jan 2018 11:00

Last Modified:

24 Oct 2019 09:06

Publisher DOI:

10.1371/journal.ppat.1006808

PubMed ID:

29287109

BORIS DOI:

10.7892/boris.108903

URI:

https://boris.unibe.ch/id/eprint/108903

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