Mycoplasma bovis co-infection with bovine viral diarrhea virus in bovine macrophages

Bürgi, Nina Simona; Josi, Christoph; Bürki, Sibylle; Schweizer, Matthias; Pilo, Paola (2018). Mycoplasma bovis co-infection with bovine viral diarrhea virus in bovine macrophages. Veterinary research, 49(1), p. 2. BioMed Central 10.1186/s13567-017-0499-1

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Several studies suggest that synergisms between Mycoplasma bovis and other microorganisms might exacerbate disease outcome of bovine mycoplasmosis. Screening several bovine cell types to assess their potential use as in vitro infection models for M. bovis, it was observed that a widely used cell line of bovine macrophages (Bomac cells) is in fact persistently infected with bovine viral diarrhea virus (BVDV). The cell line was first cured of this virus allowing comparative studies between both cell lines. Subsequently, uptake and co-culture of two M. bovis strains of different clonal complexes with Bomac cells contaminated with BVDV and in BVDV-free Bomac cells were assessed. Additionally, cell viability, cytotoxicity and induction of apoptosis after infection with M. bovis were evaluated. No differences in the levels of uptake and growth in co-culture were observed between the two Bomac cell types and both M. bovis strains. Cytotoxicity was increased after infection of BVDV-free cells with one of the two strains, while apoptotic cell death was slightly induced by this strain in both cell lines. Overall, the presence or absence of BVDV in Bomac cells did not grossly change the parameters tested upon infection with M. bovis. Nevertheless, this cell model is very useful when studying viral co-infections with bacteria and could also be used for multiple co-infections. Considering the broad contamination of cell cultures with BVDV, careful screening for this virus should routinely be performed as its presence might be relevant depending on the molecular mechanisms being investigated.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Bürgi, Nina Simona; Josi, Christoph; Bürki, Sibylle and Schweizer, Matthias

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1297-9716

Publisher:

BioMed Central

Language:

English

Submitter:

Christoph Josi

Date Deposited:

10 Apr 2018 16:25

Last Modified:

11 Apr 2018 06:31

Publisher DOI:

10.1186/s13567-017-0499-1

PubMed ID:

29316971

BORIS DOI:

10.7892/boris.108958

URI:

https://boris.unibe.ch/id/eprint/108958

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