Steinmann, Michael E.; Schmidt, Remo S.; Pereira de Macêdo, Juan; Kunz Renggli, Christina; Bütikofer, Peter; Rentsch, Doris; Mäser, Pascal; Sigel, Erwin (2017). Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei. PLoS ONE, 12(12), pp. 1-18. Public Library of Science 10.1371/journal.pone.0188219
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CLC type anion transport proteins are homo-dimeric or hetero-dimeric with an integrated transport function in each subunit. We have identified and partially characterized three members of this family named TbVCL1, TbVCL2 and TbVCL3 in Trypanosoma brucei. Among the human CLC family members, the T. brucei proteins display highest similarity to CLC-6 and CLC-7. TbVCL1, but not TbVCL2 and TbVCL3 is able to complement growth of a CLC-deficient Saccharomyces cerevisiae mutant. All TbVCL-HA fusion proteins localize intracellulary in procyclic form trypanosomes. TbVCL1 localizes close to the Golgi apparatus and TbVCL2 and TbVCL3 to the endoplasmic reticulum. Upon expression in Xenopus oocytes, all three proteins induce similar outward rectifying chloride ion currents. Currents are sensitive to low concentrations of DIDS, insensitive to the pH in the range 5.4 to 8.4 and larger in nitrate than in chloride medium.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Steinmann, Michael Eduard, Pereira de Macêdo, Juan, Bütikofer, Peter, Rentsch, Doris, Sigel, Erwin |
Subjects: |
500 Science > 580 Plants (Botany) 600 Technology > 610 Medicine & health |
ISSN: |
1932-6203 |
Publisher: |
Public Library of Science |
Language: |
English |
Submitter: |
Peter Alfred von Ballmoos-Haas |
Date Deposited: |
29 Jan 2018 11:12 |
Last Modified: |
05 Dec 2022 15:09 |
Publisher DOI: |
10.1371/journal.pone.0188219 |
PubMed ID: |
29244877 |
BORIS DOI: |
10.7892/boris.109290 |
URI: |
https://boris.unibe.ch/id/eprint/109290 |
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