Proteomic Analysis of Lung Tissue by DIGE.

Nally, Jarlath E; Schuller, Simone (2018). Proteomic Analysis of Lung Tissue by DIGE. Methods in molecular biology, 1664, pp. 167-183. Springer 10.1007/978-1-4939-7268-5_14

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Lungs perform an essential physiological function, mediated by a complex series of events that involve the coordination of multiple cell types to support not only gaseous exchange, but homeostasis and protection from infection. Guinea pigs are an important animal disease model for a number of infectious and noninfectious pulmonary conditions and the availability of a complete genome facilitates comprehensive analysis of tissues using the tools of proteomics. Here, we describe the application of 2-D Difference Gel Electrophoresis (DIGE) to compare, quantify, and identify differential protein expression of proteins in lung tissue from guinea pigs with leptospiral pulmonary hemorrhage syndrome (LPHS) compared to noninfected controls. 2-D DIGE is a powerful technique that provides novel insights into the dynamics of the complex lung proteome during health and disease.

Item Type:

Journal Article (Review Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Internal Medicine

UniBE Contributor:

Schuller, Simone

Subjects:

600 Technology > 630 Agriculture

ISSN:

1940-6029

Publisher:

Springer

Language:

English

Submitter:

Simone Schuller

Date Deposited:

20 Mar 2019 07:57

Last Modified:

20 Mar 2019 07:57

Publisher DOI:

10.1007/978-1-4939-7268-5_14

PubMed ID:

29019133

Uncontrolled Keywords:

CyDye DIGE Guinea pigs Lung Proteomics Pulmonary SDS-PAGE

URI:

https://boris.unibe.ch/id/eprint/110567

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