Proteomic Analysis of Lung Tissue by DIGE.

Nally, Jarlath E; Schuller, Simone (2018). Proteomic Analysis of Lung Tissue by DIGE. Methods in molecular biology, 1664, pp. 167-183. Springer 10.1007/978-1-4939-7268-5_14

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Lungs perform an essential physiological function, mediated by a complex series of events that involve the coordination of multiple cell types to support not only gaseous exchange, but homeostasis and protection from infection. Guinea pigs are an important animal disease model for a number of infectious and noninfectious pulmonary conditions and the availability of a complete genome facilitates comprehensive analysis of tissues using the tools of proteomics. Here, we describe the application of 2-D Difference Gel Electrophoresis (DIGE) to compare, quantify, and identify differential protein expression of proteins in lung tissue from guinea pigs with leptospiral pulmonary hemorrhage syndrome (LPHS) compared to noninfected controls. 2-D DIGE is a powerful technique that provides novel insights into the dynamics of the complex lung proteome during health and disease.

Item Type:	Journal Article (Review Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Internal Medicine
UniBE Contributor:	Schuller, Simone
Subjects:	600 Technology > 630 Agriculture
ISSN:	1940-6029
Publisher:	Springer
Language:	English
Submitter:	Simone Schuller
Date Deposited:	20 Mar 2019 07:57
Last Modified:	05 Dec 2022 15:10
Publisher DOI:	10.1007/978-1-4939-7268-5_14
PubMed ID:	29019133
Uncontrolled Keywords:	CyDye DIGE Guinea pigs Lung Proteomics Pulmonary SDS-PAGE
URI:	https://boris.unibe.ch/id/eprint/110567