High resolution tissue mass spectrometry imaging reveals a refined functional anatomy of the human adult adrenal gland.

Sun, Na; Wu, Yin; Nanba, Kazutaka; Sbiera, Silviu; Kircher, Stefan; Kunzke, Thomas; Aichler, Michaela; Berezowska, Sabina Anna; Reibetanz, Joachim; Rainey, William E; Fassnacht, Martin; Walch, Axel; Kroiss, Matthias (2018). High resolution tissue mass spectrometry imaging reveals a refined functional anatomy of the human adult adrenal gland. Endocrinology, 159(3), pp. 1511-1524. Oxford University Press 10.1210/en.2018-00064

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In the adrenal gland, neuroendocrine cells that synthesize catecholamines and epithelial cells that produce steroid hormones are united beneath a common organ capsule to function as a single stress-responsive organ. The functional anatomy of the steroid hormone producing adrenal cortex and the catecholamine producing medulla is ill defined at the level of small molecules. Here, we report the first comprehensive high-resolution mass spectrometry imaging (MSI) map of the normal human adrenal gland. A large variety of biomolecules was accessible by matrix-assisted laser desorption/ionization-Fourier transform-ion cyclotron resonance (MALDI-FT-ICR) MSI, including nucleoside phosphates indicative of oxidative phosphorylation, sterol and steroid metabolites, intermediates of glycolysis and the tricarboxylic acid (TCA) cycle, lipids and fatty acids. Statistical clustering analyses yielded a molecular defined adrenal anatomy of ten distinct molecular zones including a highly structured corticomedullary interface. By incorporating pathway information, activities of carbohydrate, amino acid and lipid metabolism as well as endocrine bioactivity were revealed to be highly spatially organized which could be visualized as different molecularly defined zones. Together, these findings provide a molecular definition of human adult adrenal gland structure beyond classical histological anatomy.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Berezowska, Sabina Anna

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1945-7170

Publisher:

Oxford University Press

Language:

English

Submitter:

Sabina Anna Berezowska

Date Deposited:

02 Feb 2018 14:17

Last Modified:

08 Nov 2018 01:30

Publisher DOI:

10.1210/en.2018-00064

PubMed ID:

29385420

URI:

https://boris.unibe.ch/id/eprint/110746

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