Mesenchymal stromal cells from umbilical cord Wharton's jelly trigger oligodendroglial differentiation in neural progenitor cells through cell-to-cell contact.

Oppliger, Byron; Jörger Messerli, Marianne; Simillion, Cedric André Marie; Müller, Martin; Surbek, Daniel; Schoeberlein, Andreina (2017). Mesenchymal stromal cells from umbilical cord Wharton's jelly trigger oligodendroglial differentiation in neural progenitor cells through cell-to-cell contact. Cytotherapy, 19(7), pp. 829-838. Elsevier 10.1016/j.jcyt.2017.03.075

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BACKGROUND AIMS Wharton's jelly mesenchymal stromal cells (WJ-MSCs) might be ideal candidates to treat perinatal brain damage. Their secretome has been shown to have beneficial effects on neuroregeneration, in part through interaction with neural progenitor cells (NPCs). However, it remains unclear whether cell-to-cell contact decisively contributes to this positive effect. The objective of this study was to elucidate the mechanism through which differentiation in NPCs is triggered after exposure to WJ-MSCs. Furthermore, given that WJ-MSCs can be derived from term (tWJ-MSCs) or preterm (ptWJ-MSCs) deliveries and that WJ-MSCs might be used for transplantations independent of gestational age, the influence of tWJ-MSCs versus ptWJ-MSCs on the differentiation capacities of NPCs was studied. METHODS The effect of tWJ-MSCs and ptWJ-MSCs on the expression of neuroglial markers in NPCs was assessed in co-culture (CC), conditioned medium (CM) or transwell CC experiments by immunocytochemistry, real-time polymerase chain reaction and Western blot. Additionally, mass spectrometry was used to study their secretomes. RESULTS NPCs showed an increased expression of glial markers after CC with WJ-MSCs or exposure to WJ-MSC-CMs. CC had a more prominent effect on the expression of glial markers compared with CM or transwell CCs. tWJ-MSCs more strongly induced the expression of mature oligodendroglial markers compared with ptWJ-MSCs. A possible role in enhancing this maturation could be attributed to the laminin α2-subunit. CONCLUSIONS Cell-to-cell contact between WJ-MSCs and NPCs induces oligodendrogenesis on NPCs, whereas trophic factor secretion is sufficient to promote astrogenesis. Thus, transplanting WJ-MSCs may promote endogenous neuroregeneration in perinatal brain damage.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Oppliger, Byron; Jörger Messerli, Marianne; Simillion, Cedric André Marie; Müller, Martin; Surbek, Daniel and Schoeberlein, Andreina

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1465-3249

Publisher:

Elsevier

Funders:

[UNSPECIFIED] Cryo-Save, Switzerland
[UNSPECIFIED] Eagle Foundation, Crans-près-Celigny, Switzerland
[UNSPECIFIED] Mobiliar Jubilaumsstiftung, Switzerland
[UNSPECIFIED] Forschungs-Grant Inselspital Bern, Switzerland

Language:

English

Submitter:

Monika Zehr

Date Deposited:

20 Mar 2018 11:58

Last Modified:

27 Mar 2019 11:26

Publisher DOI:

10.1016/j.jcyt.2017.03.075

PubMed ID:

28457739

Web of Science ID:

000403976600006

Uncontrolled Keywords:

Wharton's jelly mesenchymal stromal cells neural progenitor cells neuroregeneration oligodendrogenesis

BORIS DOI:

10.7892/boris.110784

URI:

https://boris.unibe.ch/id/eprint/110784

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