Oxidation-sensitive polymersomes as vaccine nanocarriers enhance humoral responses against Lassa virus envelope glycoprotein.

Galan-Navarro, Clara; Rincon-Restrepo, Marcela; Zimmer, Gert; Ollmann Saphire, Erica; Hubbell, Jeffrey A; Hirosue, Sachiko; Swartz, Melody A; Kunz, Stefan (2017). Oxidation-sensitive polymersomes as vaccine nanocarriers enhance humoral responses against Lassa virus envelope glycoprotein. Virology, 512, pp. 161-171. Academic Press 10.1016/j.virol.2017.09.013

[img] Text
1-s2.0-S0042682217303227-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Lassa virus (LASV) causes severe hemorrhagic fever with high mortality, yet no vaccine currently exists. Antibodies targeting viral attachment proteins are crucial for protection against many viral infections. However, the envelope glycoprotein (GP)-1 of LASV elicits weak antibody responses due to extensive glycan shielding. Here, we explored a novel vaccine strategy to enhance humoral immunity against LASV GP1. Using structural information, we designed a recombinant GP1 immunogen, and then encapsulated it into oxidation-sensitive polymersomes (PS) as nanocarriers that promote intracellular MHCII loading. Mice immunized with adjuvanted PS (LASV GP1) showed superior humoral responses than free LASV GP1, including antibodies with higher binding affinity to virion GP1, increased levels of polyfunctional anti-viral CD4 T cells, and IgG-secreting B cells. PS (LASV GP1) elicited a more diverse epitope repertoire of anti-viral IgG. Together, these data demonstrate the potential of our nanocarrier vaccine platform for generating virus-specific antibodies against weakly immunogenic viral antigens.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Zimmer, Gert

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0042-6822

Publisher:

Academic Press

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

15 May 2018 14:16

Last Modified:

15 May 2018 14:25

Publisher DOI:

10.1016/j.virol.2017.09.013

PubMed ID:

28963882

Uncontrolled Keywords:

Anti-viral antibodies Lassa virus Nanocarrier Polymersomes Subunit vaccine

BORIS DOI:

10.7892/boris.112049

URI:

https://boris.unibe.ch/id/eprint/112049

Actions (login required)

Edit item Edit item
Provide Feedback