Increased motility of mesenchymal stem cells is correlated with inhibition of stimulated peripheral blood mononuclear cells.

Bertolo, Alessandro; Pavlicek, David; Gemperli, Armin; Baur, Martin; Pötzel, Tobias; Stoyanov, Jivko (2017). Increased motility of mesenchymal stem cells is correlated with inhibition of stimulated peripheral blood mononuclear cells. Journal of stem cells & regenerative medicine, 13(2), pp. 62-74. G.N. Corporation

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Immunomodulatory properties of mesenchymal stem cells (MSC) are key components of their successful applications in clinical setting. However, treatments based on MSC immunomodulation need understanding of cell characteristics before cell transplantation. We used live-imaging to test the suitability of the MSC motility as a parameter for quick prediction of the immunomodulatory potential of human MSC in regulating the activity of stimulated peripheral blood mononuclear cells (PBMC). Bone marrow MSC, from various donors andpassages, were cultured with or without stimulated PBMC. After seven days, immunomodulation was assessed by measuring PBMC proliferation, IgG production and cytokine secretion in MSC and PBMC monocultures and co-cultures, and results were correlated to MSC motility. In co-culture, we observed that MSC successfully inhibited PBMC activity, reducing PBMC proliferation and IgG production compared to PBMC monoculture. MSC modulated PBMC to reduce the secretion of TNFα and IL-10, increase IL-6, G-CSF and MCP-1, while GM-CSF was not affected. By live-imaging tracking of cell trajectories, we observed that fast moving MSC were inhibiting more efficiently stimulated PBMC compared to slow ones. In co-culture, fast MSC were more effective in inhibiting IgG production (˜30% less IgG), and secreted higher levels of IL-10 (˜10% increase) and GM-CSF (˜20% increase) compared to slower cells. Furthermore, fast MSC in monocultures produced 2.3-fold more IL-6, 1.5-fold MCP-1 and 1.2-fold G-CSF in comparison to slower cells. In conclusion, live-imaging cell tracking allowed us to develop an indicative assay of the immune-regulatory potential of MSC prior toadministration. Key Words: Human mesenchymal stem cells, Immunomodulatory potential, In vitro cell motility, Stem cell transplantation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Stoyanov, Jivko

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0973-7154

Publisher:

G.N. Corporation

Language:

English

Submitter:

Marlene Wolf Tobler

Date Deposited:

28 Mar 2018 15:20

Last Modified:

29 Mar 2018 06:33

Related URLs:

PubMed ID:

29391751

Uncontrolled Keywords:

Human mesenchymal stem cells Immunomodulatory potential In vitro cell motility Stem cell transplantation

BORIS DOI:

10.7892/boris.112544

URI:

https://boris.unibe.ch/id/eprint/112544

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