Boehringer, Stephan; Ruzgys, Paulius; Tamò, Luca Giuseppe Athos; Šatkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq; Hradetzky, David (2018). A new electrospray method for targeted gene delivery. Scientific Reports, 8(1), p. 4031. Nature Publishing Group 10.1038/s41598-018-22280-2
|
Text
Boehringer SciRep 2018.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (2MB) | Preview |
A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Ruzgys, Paulius, Tamò, Luca Giuseppe Athos, Geiser, Thomas (A), Gazdhar, Amiq |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2045-2322 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Tanya Karrer |
Date Deposited: |
17 Apr 2018 10:49 |
Last Modified: |
29 Mar 2023 23:35 |
Publisher DOI: |
10.1038/s41598-018-22280-2 |
PubMed ID: |
29507307 |
Additional Information: |
Boehringer, Ruzgys, Gasdhar and Hradetzky contributed equally to this work |
BORIS DOI: |
10.7892/boris.112618 |
URI: |
https://boris.unibe.ch/id/eprint/112618 |
Actions (login required)
 |
Edit item |