A new electrospray method for targeted gene delivery.

Boehringer, Stephan; Ruzgys, Paulius; Tamò, Luca Giuseppe Athos; Šatkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq; Hradetzky, David (2018). A new electrospray method for targeted gene delivery. Scientific Reports, 8(1), p. 4031. Nature Publishing Group 10.1038/s41598-018-22280-2

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A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Ruzgys, Paulius; Tamò, Luca Giuseppe Athos; Geiser, Thomas and Gazdhar, Amiq

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Tanya Karrer

Date Deposited:

17 Apr 2018 10:49

Last Modified:

03 Jan 2019 16:25

Publisher DOI:

10.1038/s41598-018-22280-2

PubMed ID:

29507307

Additional Information:

Boehringer, Ruzgys, Gasdhar and Hradetzky contributed equally to this work

BORIS DOI:

10.7892/boris.112618

URI:

https://boris.unibe.ch/id/eprint/112618

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