Elevating serotonin pre-partum alters the Holstein dairy cow hepatic adaptation to lactation.

Weaver, Samantha R; Prichard, Allan S; Maerz, Noah L; Prichard, Austin P; Endres, Elizabeth Lynne; Hernandez Castellano, Lorenzo Enrique; Akins, Matthew S; Bruckmaier, Rupert; Hernandez, Laura L (2017). Elevating serotonin pre-partum alters the Holstein dairy cow hepatic adaptation to lactation. PLoS ONE, 12(9), e0184939. Public Library of Science 10.1371/journal.pone.0184939

[img]
Preview
Text
journal.pone.0184939.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (7MB) | Preview

Serotonin is known to regulate energy and calcium homeostasis in several mammalian species. The objective of this study was to determine if pre-partum infusions of 5-hydroxytryptophan (5-HTP), the immediate precursor to serotonin synthesis, could modulate energy homeostasis at the level of the hepatocyte in post-partum Holstein and Jersey dairy cows. Twelve multiparous Holstein cows and twelve multiparous Jersey cows were intravenously infused daily for approximately 7 d pre-partum with either saline or 1 mg/kg bodyweight of 5-HTP. Blood was collected for 14 d post-partum and on d30 post-partum. Liver biopsies were taken on d1 and d7 post-partum. There were no changes in the circulating concentrations of glucose, insulin, glucagon, non-esterified fatty acids, or urea nitrogen in response to treatment, although there were decreased beta-hydroxybutyrate concentrations with 5-HTP treatment around d6 to d10 post-partum, particularly in Jersey cows. Cows infused with 5-HTP had increased hepatic serotonin content and increased mRNA expression of the serotonin 2B receptor on d1 and d7 post-partum. Minimal changes were seen in the hepatic mRNA expression of various gluconeogenic enzymes. There were no changes in the mRNA expression profile of cell-cycle progression marker cyclin-dependent kinase 4 or apoptotic marker caspase 3, although proliferating cell nuclear antigen expression tended to be increased in Holstein cows infused with 5-HTP on d1 post-partum. Immunofluorescence assays showed an increased number of CASP3- and Ki67-positive cells in Holstein cows infused with 5-HTP on d1 post-partum. Given the elevated hepatic serotonin content and increased mRNA abundance of 5HTR2B, 5-HTP infusions may be stimulating an autocrine-paracrine adaptation to lactation in the Holstein cow liver.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Endres, Elizabeth Lynne; Hernandez Castellano, Lorenzo Enrique and Bruckmaier, Rupert

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Hélène Elisabeth Meier

Date Deposited:

19 Jun 2018 16:18

Last Modified:

22 Oct 2019 22:54

Publisher DOI:

10.1371/journal.pone.0184939

PubMed ID:

28922379

BORIS DOI:

10.7892/boris.114684

URI:

https://boris.unibe.ch/id/eprint/114684

Actions (login required)

Edit item Edit item
Provide Feedback