A New Multiantigen Immunoassay for the Quantification of IgG Antibodies to Capsular Polysaccharides of Streptococcus pneumoniae

Roth, Felix; Burkart, Thomas; Mühlemann, Kathrin (1997). A New Multiantigen Immunoassay for the Quantification of IgG Antibodies to Capsular Polysaccharides of Streptococcus pneumoniae. Journal of infectious diseases, 176(2), pp. 526-529. The University of Chicago Press 10.1086/517281

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A new nitrocellulose-based solid-phase multiantigen immunoassay (MAIA) for the detection of serum antibodies to Streptococcus pneumoniae capsular polysaccharides (PPSs) is presented. Evaluation with human sera showed that the MAIA test is reproducible, sensitive, and specific. It correlated well with a conventional ELISA method. The multiantigen strip system allowed quantification of antibodies against several PPS serotypes simultaneously and with a minimal amount of serum specimen. The presented solid-phase immunoassay for the quantification of anti-PPS antibodies seems to be a superior and attractive alternative to currently used ELISA tests and offers possibilities for standardization.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Burkart, Thomas and Mühlemann, Kathrin

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0022-1899

Publisher:

The University of Chicago Press

Language:

English

Submitter:

Marceline Brodmann

Date Deposited:

08 Oct 2020 11:43

Last Modified:

08 Oct 2020 11:43

Publisher DOI:

10.1086/517281

BORIS DOI:

10.7892/boris.115601

URI:

https://boris.unibe.ch/id/eprint/115601

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