Farine, Emilie; Egle, Manuel; Boone, Alice C; Christensen, Sandro; Carrel, Thierry; Tevaearai Stahel, Hendrik T; Henning Longnus, Sarah (2018). Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. PLoS ONE, 13(4), e0195721. Public Library of Science 10.1371/journal.pone.0195721
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BACKGROUND
Ex vivo heart perfusion systems, allowing continuous perfusion of the coronary vasculature, have recently been introduced to limit ischemic time of donor hearts prior to transplantation. Hearts are, however, perfused in an unloaded manner (via the aorta) and therefore, cardiac contractile function cannot be reliably evaluated.
OBJECTIVES
We aim to develop a ventricular loading device that enables monitoring of myocardial function in an ex vivo perfusion system. In this initial study, was to develop a prototype for rat experimentation.
METHODS
We designed a device consisting of a ventricular balloon and a reservoir balloon, connected through an electronic check valve, which opens and closes in coordination with changes in ventricular pressure. All balloons were produced in our laboratory and their properties, particularly pressure-volume relationships, were characterized. We developed a mock ventricle in vitro test system to evaluate the device, which was ultimately tested in ex vivo perfused rat hearts.
RESULTS
Balloon production was consistent and balloon properties were maintained over time and with use on the device. Results from in vitro and ex vivo experiments show that the device functions appropriately; hemodynamic function can be measured and compares well to measurements made in an isolated, working (loaded) rat heart preparation.
CONCLUSIONS
Our cardiac loading device appears to reliably allow measurement of several left ventricular hemodynamic parameters and provides the opportunity to control ventricular load.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Heart Surgery 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie |
UniBE Contributor: |
Farine, Emilie, Egle, Manuel, Christensen, Sandro, Carrel, Thierry, Henning Longnus, Sarah |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1932-6203 |
Publisher: |
Public Library of Science |
Language: |
English |
Submitter: |
Daniela Huber |
Date Deposited: |
04 Jun 2018 13:07 |
Last Modified: |
27 Feb 2024 14:28 |
Publisher DOI: |
10.1371/journal.pone.0195721 |
PubMed ID: |
29702648 |
BORIS DOI: |
10.7892/boris.116915 |
URI: |
https://boris.unibe.ch/id/eprint/116915 |
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