Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion.

Farine, Emilie; Egle, Manuel; Boone, Alice C; Christensen, Sandro; Carrel, Thierry; Tevaearai Stahel, Hendrik T; Henning Longnus, Sarah (2018). Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. PLoS ONE, 13(4), e0195721. Public Library of Science 10.1371/journal.pone.0195721

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BACKGROUND Ex vivo heart perfusion systems, allowing continuous perfusion of the coronary vasculature, have recently been introduced to limit ischemic time of donor hearts prior to transplantation. Hearts are, however, perfused in an unloaded manner (via the aorta) and therefore, cardiac contractile function cannot be reliably evaluated. OBJECTIVES We aim to develop a ventricular loading device that enables monitoring of myocardial function in an ex vivo perfusion system. In this initial study, was to develop a prototype for rat experimentation. METHODS We designed a device consisting of a ventricular balloon and a reservoir balloon, connected through an electronic check valve, which opens and closes in coordination with changes in ventricular pressure. All balloons were produced in our laboratory and their properties, particularly pressure-volume relationships, were characterized. We developed a mock ventricle in vitro test system to evaluate the device, which was ultimately tested in ex vivo perfused rat hearts. RESULTS Balloon production was consistent and balloon properties were maintained over time and with use on the device. Results from in vitro and ex vivo experiments show that the device functions appropriately; hemodynamic function can be measured and compares well to measurements made in an isolated, working (loaded) rat heart preparation. CONCLUSIONS Our cardiac loading device appears to reliably allow measurement of several left ventricular hemodynamic parameters and provides the opportunity to control ventricular load.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie

UniBE Contributor:

Farine, Emilie; Egle, Manuel; Christensen, Sandro; Carrel, Thierry and Henning Longnus, Sarah

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Daniela Huber

Date Deposited:

04 Jun 2018 13:07

Last Modified:

04 Jun 2018 13:07

Publisher DOI:

10.1371/journal.pone.0195721

PubMed ID:

29702648

BORIS DOI:

10.7892/boris.116915

URI:

https://boris.unibe.ch/id/eprint/116915

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