Full-genome based molecular characterization of encephalitis-associated bovine astroviruses

Bouzalas, Ilias; Wüthrich, Daniel; Selimovic-Hamza, Senija; Drögemüller, Cord; Bruggmann, Rémy; Seuberlich, Torsten (2016). Full-genome based molecular characterization of encephalitis-associated bovine astroviruses. Infection, genetics and evolution, 44, pp. 162-168. Elsevier 10.1016/j.meegid.2016.06.052

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Novel types of astrovirus have been identified recently in association with neurological disease in cattle. Among those viruses is bovine astrovirus CH13 (BoAstV CH13) that has been identified in Switzerland in a cow with encephalitis. Molecular testing by a combination of reverse transcription (RT-) PCR and in situ hybridization (ISH) indicated that astrovirus infection accounts for around one quarter of viral encephalitis cases of unknown etiology in cattle. Yet, it remained to be explored whether these animals were infected by BoAstV CH13 or other astrovirus species. In the present study we sequenced the entire astrovirus genome in brain tissues of eight RT-PCR and/or ISH positive cattle. Phylogenetic comparison of the genomic RNA and the encoded non-structural and structural proteins revealed that all these astrovirus strains were very similar to BoAstV CH13 as well as to a bovine encephalitis strain reported from the USA (BoAstV NeuroS1), and clearly distinct from other previously reported astroviruses. Conserved 5' and 3' untranslated regions (UTRs) were predicted to display distinct secondary RNA structures, which likely play a role in viral RNA replication and/or protein translation. Based on these data we propose that BoAstV CH13/NeuroS1 represents a new genotype species within the genus Mammastrovirus. The high degree of similarity between the strains and their relative distance to other genotype species suggest that during evolution some astroviruses acquired factors that specifically contribute to neuroinvasion.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Genomics

UniBE Contributor:

Bouzalas, Ilias; Wüthrich, Daniel; Selimovic-Hamza, Senija; Drögemüller, Cord; Bruggmann, Rémy and Seuberlich, Torsten

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

1567-1348

Publisher:

Elsevier

Language:

English

Submitter:

Torsten Seuberlich

Date Deposited:

07 Jun 2018 09:27

Last Modified:

07 Jun 2018 09:36

Publisher DOI:

10.1016/j.meegid.2016.06.052

PubMed ID:

27378415

BORIS DOI:

10.7892/boris.117046

URI:

https://boris.unibe.ch/id/eprint/117046

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