Downregulation of Autophagy-Related Proteins 1, 5, and 16 in Testicular Germ Cell Tumors Parallels Lowered LC3B and Elevated p62 Levels, Suggesting Reduced Basal Autophagy.

Liu, He; He, Zhaoyue; Bode, Peter; Moch, Holger; Simon, Hans-Uwe (2018). Downregulation of Autophagy-Related Proteins 1, 5, and 16 in Testicular Germ Cell Tumors Parallels Lowered LC3B and Elevated p62 Levels, Suggesting Reduced Basal Autophagy. Frontiers in oncology, 8(366), p. 366. Frontiers Research Foundation 10.3389/fonc.2018.00366

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Autophagy is a cellular "self-digestion" process known to be essential for various physiological and pathological pathways, including cancer, where its role appears to be context-dependent. In this work, we aimed to investigate the level of autophagy by evaluating the expression of key autophagy-related proteins (ATGs) in testicular germ cell tumors (TGCT) for which autophagy has been rarely investigated. We decided to use an immunohistochemical (IHC) staining approach employing a tissue microarray (TMA). Software-based evaluation of the integrated optical densities (IODs) of these proteins indicated a significant downregulation of ATG1, ATG5, and ATG16L1. Accordingly, reduced levels of microtubule-associated proteins 1A/1B light chain 3B (LC3B) were found to parallel increases in sequestosome-1 (SQSTM1 or p62), a protein normally degraded via autophagy, suggesting an reduction in autophagy with TGCT. Thus, our work provides evidence for a tumor suppressive function of autophagy in the development of TGCT and supports the concept of a context-dependent role of autophagy in tumorigenesis which is tumor type-dependent.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

Liu, He; He, Zhaoyue and Simon, Hans-Uwe

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2234-943X

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Celine Joray

Date Deposited:

26 Sep 2018 10:12

Last Modified:

01 Dec 2020 15:20

Publisher DOI:

10.3389/fonc.2018.00366

PubMed ID:

30245976

Uncontrolled Keywords:

ATGs LC3B autophagy p62 testicular germ cell tumor (TGCT) tumor suppression

BORIS DOI:

10.7892/boris.120122

URI:

https://boris.unibe.ch/id/eprint/120122

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