Bischerour, Julien; Bhullar, Simran; Denby Wilkes, Cyril; Régnier, Vinciane; Mathy, Nathalie; Dubois, Emeline; Singh, Aditi; Swart, Estienne Carl; Arnaiz, Olivier; Sperling, Linda; Nowacki, Mariusz; Bétermier, Mireille (2018). Six domesticated PiggyBac transposases together carry out programmed DNA elimination in . eLife, 7 eLife Sciences Publications 10.7554/eLife.37927
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The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In , PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision complex embedding Pgm. PgmL depletion can increase erroneous targeting of residual Pgm-mediated DNA cleavage, indicating that PgmLs contribute to accurately position the complex on IES ends. DNA rearrangements in constitute a rare example of a biological process jointly managed by six distinct domesticated transposases.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate 08 Faculty of Science > Department of Biology > Institute of Cell Biology |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Bhullar, Simran, Singh, Aditi, Swart, Estienne Carl, Nowacki, Mariusz |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2050-084X |
Publisher: |
eLife Sciences Publications |
Language: |
English |
Submitter: |
Mariusz Nowacki |
Date Deposited: |
26 Sep 2018 10:02 |
Last Modified: |
05 Dec 2022 15:18 |
Publisher DOI: |
10.7554/eLife.37927 |
PubMed ID: |
30223944 |
Uncontrolled Keywords: |
DNA elimination IES chromosomes ciliates gene expression piggyBacv transposées domestication |
BORIS DOI: |
10.7892/boris.120124 |
URI: |
https://boris.unibe.ch/id/eprint/120124 |
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