Six domesticated PiggyBac transposases together carry out programmed DNA elimination in .

Bischerour, Julien; Bhullar, Simran; Denby Wilkes, Cyril; Régnier, Vinciane; Mathy, Nathalie; Dubois, Emeline; Singh, Aditi; Swart, Estienne Carl; Arnaiz, Olivier; Sperling, Linda; Nowacki, Mariusz; Bétermier, Mireille (2018). Six domesticated PiggyBac transposases together carry out programmed DNA elimination in . eLife, 7 eLife Sciences Publications 10.7554/eLife.37927

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The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In , PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision complex embedding Pgm. PgmL depletion can increase erroneous targeting of residual Pgm-mediated DNA cleavage, indicating that PgmLs contribute to accurately position the complex on IES ends. DNA rearrangements in constitute a rare example of a biological process jointly managed by six distinct domesticated transposases.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Bhullar, Simran; Singh, Aditi; Swart, Estienne Carl and Nowacki, Mariusz

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2050-084X

Publisher:

eLife Sciences Publications

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

26 Sep 2018 10:02

Last Modified:

27 Oct 2019 16:23

Publisher DOI:

10.7554/eLife.37927

PubMed ID:

30223944

Uncontrolled Keywords:

DNA elimination IES chromosomes ciliates gene expression piggyBacv transposées domestication

BORIS DOI:

10.7892/boris.120124

URI:

https://boris.unibe.ch/id/eprint/120124

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