Monitoring of cefepime in urine by micellar electrokinetic capillary chromatography with ultraviolet detection and liquid chromatography coupled to mass spectrometry.

Kummer, Mirjam; Šestáková, Nela; Theurillat, Regula; Huynh-Do, Uyen; Endimiani, Andrea; Sendi, Parham; Thormann, Wolfgang (2018). Monitoring of cefepime in urine by micellar electrokinetic capillary chromatography with ultraviolet detection and liquid chromatography coupled to mass spectrometry. Journal of separation science, 41(21), pp. 4067-4074. Wiley-VCH 10.1002/jssc.201800763

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Cefepime monitoring in urine by micellar electrokinetic capillary chromatography with UV detection and liquid chromatography coupled to mass spectrometry via electrospray ionization is described. For micellar electrokinetic capillary chromatography, sample preparation comprised urine dilution and dodecyl-sulfate protein precipitation at pH 4.5, whereas diluted urines were analyzed in the other assay. Both approaches provided suitable conditions for cefepime analysis in urines of healthy volunteers that were spiked with cefepime. Cefepime monitoring by micellar electrokinetic capillary chromatography in samples from patients taking multiple drugs were prone to interferences, whereas liquid chromatography coupled to mass spectrometry provided clean chromatograms and thus selective detection of cefepime in all samples. The latter assay was used to measure urinary cefepime in a prospective pilot study and to assess cefepime stability in urines at 25, 4, -20 and -70°C. The data suggest that urinary cefepime is stable for at least 72 h at all tested temperatures.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology

UniBE Contributor:

Endimiani, Andrea; Sendi, Parham and Thormann, Wolfgang

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1615-9306

Publisher:

Wiley-VCH

Language:

English

Submitter:

Wolfgang Thormann

Date Deposited:

08 Oct 2018 08:52

Last Modified:

17 Nov 2019 08:39

Publisher DOI:

10.1002/jssc.201800763

PubMed ID:

30198147

Uncontrolled Keywords:

capillary electrophoresis cefepime micellar electrokinetic capillary chromatography therapeutic drug monitoring

BORIS DOI:

10.7892/boris.120356

URI:

https://boris.unibe.ch/id/eprint/120356

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