Singh, Aditi; Vancura, Adrienne; Woycicki, Rafal K.; Hogan, Daniel J; Hendrick, Alan G; Nowacki, Mariusz (2018). Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. PLoS ONE, 13(10), e0206667. Public Library of Science 10.1371/journal.pone.0206667
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5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methylcytosine DNA methylation as an integral part of its epigenetic arsenal.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate 08 Faculty of Science > Department of Biology > Institute of Cell Biology |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Singh, Aditi, Woycicki, Rafal, Nowacki, Mariusz |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1932-6203 |
Publisher: |
Public Library of Science |
Language: |
English |
Submitter: |
Mariusz Nowacki |
Date Deposited: |
01 Nov 2018 15:06 |
Last Modified: |
05 Dec 2022 15:18 |
Publisher DOI: |
10.1371/journal.pone.0206667 |
PubMed ID: |
30379964 |
BORIS DOI: |
10.7892/boris.120859 |
URI: |
https://boris.unibe.ch/id/eprint/120859 |
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