Determination of the presence of 5-methylcytosine in Paramecium tetraurelia.

Singh, Aditi; Vancura, Adrienne; Woycicki, Rafal K.; Hogan, Daniel J; Hendrick, Alan G; Nowacki, Mariusz (2018). Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. PLoS ONE, 13(10), e0206667. Public Library of Science 10.1371/journal.pone.0206667

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5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methylcytosine DNA methylation as an integral part of its epigenetic arsenal.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Singh, Aditi; Woycicki, Rafal and Nowacki, Mariusz

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

01 Nov 2018 15:06

Last Modified:

22 Oct 2019 19:32

Publisher DOI:

10.1371/journal.pone.0206667

PubMed ID:

30379964

BORIS DOI:

10.7892/boris.120859

URI:

https://boris.unibe.ch/id/eprint/120859

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