Inflammasomes make the case for littermate-controlled experimental design in studying host-microbiota interactions.

Mamantopoulos, Michail; Ronchi, Francesca; McCoy, Kathy D; Wullaert, Andy (2018). Inflammasomes make the case for littermate-controlled experimental design in studying host-microbiota interactions. Gut microbes, 9(4), pp. 374-381. Landes Bioscience 10.1080/19490976.2017.1421888

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Several human diseases are thought to evolve due to a combination of host genetic mutations and environmental factors that include alterations in intestinal microbiota composition termed dysbiosis. Although in some cases, host genetics may shape the gut microbiota and enable it to provoke disease, experimentally disentangling cause and consequence in such host-microbe interactions requires strict control over non-genetic confounding factors. Mouse genetic studies previously proposed Nlrp6/ASC inflammasomes as innate immunity regulators of the intestinal ecosystem. In contrast, using littermate-controlled experimental setups, we recently showed that Nlrp6/ASC inflammasomes do not alter the gut microbiota composition. Our analyses indicated that maternal inheritance and long-term separate housing are non-genetic confounders that preclude the use of non-littermate mice when analyzing host genetic effects on intestinal ecology. Here, we summarize and discuss our gut microbiota analyses in inflammasome-deficient mice for illustrating the importance of littermate experimental design in studying host-microbiota interactions.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie

UniBE Contributor:

Ronchi, Francesca

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1949-0976

Publisher:

Landes Bioscience

Language:

English

Submitter:

Thi Thao Anh Pham

Date Deposited:

04 Feb 2019 09:40

Last Modified:

02 Nov 2019 16:02

Publisher DOI:

10.1080/19490976.2017.1421888

PubMed ID:

29672197

Uncontrolled Keywords:

ASC DSS colitis Nlrp6 caspase-1 dysbiosis gut microbiota inflammasomes innate immunity interleukin littermates

BORIS DOI:

10.7892/boris.122995

URI:

https://boris.unibe.ch/id/eprint/122995

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