Mamantopoulos, Michail; Ronchi, Francesca; McCoy, Kathy D; Wullaert, Andy (2018). Inflammasomes make the case for littermate-controlled experimental design in studying host-microbiota interactions. Gut microbes, 9(4), pp. 374-381. Landes Bioscience 10.1080/19490976.2017.1421888
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Several human diseases are thought to evolve due to a combination of host genetic mutations and environmental factors that include alterations in intestinal microbiota composition termed dysbiosis. Although in some cases, host genetics may shape the gut microbiota and enable it to provoke disease, experimentally disentangling cause and consequence in such host-microbe interactions requires strict control over non-genetic confounding factors. Mouse genetic studies previously proposed Nlrp6/ASC inflammasomes as innate immunity regulators of the intestinal ecosystem. In contrast, using littermate-controlled experimental setups, we recently showed that Nlrp6/ASC inflammasomes do not alter the gut microbiota composition. Our analyses indicated that maternal inheritance and long-term separate housing are non-genetic confounders that preclude the use of non-littermate mice when analyzing host genetic effects on intestinal ecology. Here, we summarize and discuss our gut microbiota analyses in inflammasome-deficient mice for illustrating the importance of littermate experimental design in studying host-microbiota interactions.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie |
UniBE Contributor: |
Ronchi, Francesca |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1949-0976 |
Publisher: |
Landes Bioscience |
Language: |
English |
Submitter: |
Thi Thao Anh Pham |
Date Deposited: |
04 Feb 2019 09:40 |
Last Modified: |
22 May 2023 21:32 |
Publisher DOI: |
10.1080/19490976.2017.1421888 |
PubMed ID: |
29672197 |
Uncontrolled Keywords: |
ASC DSS colitis Nlrp6 caspase-1 dysbiosis gut microbiota inflammasomes innate immunity interleukin littermates |
BORIS DOI: |
10.7892/boris.122995 |
URI: |
https://boris.unibe.ch/id/eprint/122995 |