DNA methylation patterns of the S100A14, POU2F3 and SFN genes in equine sarcoid tissues.

Semik-Gurgul, E; Ząbek, T; Fornal, A; Wnuk, M; Pawlina-Tyszko, K; Gurgul, A; Klukowska-Rötzler, J.; Koch, C.; Mählmann, K; Bugno-Poniewierska, M (2018). DNA methylation patterns of the S100A14, POU2F3 and SFN genes in equine sarcoid tissues. Research in veterinary science, 119, pp. 302-307. Association for Veterinary Teaching and Research Work 10.1016/j.rvsc.2018.07.006

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Genetic and epigenetic alterations in the equine sarcoid, a locally invasive skin tumour of equids, are still poorly characterized. Numerous studies have provided reliable evidence for the relationship between the development of cancer and the loss of function of a number of tumour suppressor genes. In the present study, we assessed methylation levels in the promoter region of SFN, S100A14 and POU2F3 genes in sarcoid samples to clarify whether DNA methylation may be associated with previously identified changes in the expression level of these genes during the course of tumour progression. Using bisulfite sequencing and clone sequencing, we detected that lesional samples had a significantly higher rate of DNA methylation in the analyzed S100A14A region than the corresponding normal skin tissue. A frequent methylation of the SFN and POU2F3 promoter sequences were observed in both the tumour samples and the control skin tissues. Further studies are needed to evaluate the role of aberrant methylation in sarcoid progression and to understand the mechanisms involved in reduced expression of SFN, S100A14 and POU2F3 genes in the lesional tissues.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)

UniBE Contributor:

Klukowska-Rötzler, Jolanta and Koch, Christoph

Subjects:

600 Technology > 630 Agriculture

ISSN:

0034-5288

Publisher:

Association for Veterinary Teaching and Research Work

Language:

English

Submitter:

Andrea Gassmann-Suter

Date Deposited:

23 Jan 2019 11:37

Last Modified:

08 Feb 2019 09:12

Publisher DOI:

10.1016/j.rvsc.2018.07.006

Related URLs:

PubMed ID:

30086514

Uncontrolled Keywords:

DNA methylation Equine sarcoids POU2F3 S100A14 SFN Tumorigenesis

BORIS DOI:

10.7892/boris.123239

URI:

https://boris.unibe.ch/id/eprint/123239

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