Semik-Gurgul, E; Ząbek, T; Fornal, A; Wnuk, M; Pawlina-Tyszko, K; Gurgul, A; Klukowska-Rötzler, J.; Koch, C.; Mählmann, K; Bugno-Poniewierska, M (2018). DNA methylation patterns of the S100A14, POU2F3 and SFN genes in equine sarcoid tissues. Research in veterinary science, 119, pp. 302-307. Association for Veterinary Teaching and Research Work 10.1016/j.rvsc.2018.07.006
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Genetic and epigenetic alterations in the equine sarcoid, a locally invasive skin tumour of equids, are still poorly characterized. Numerous studies have provided reliable evidence for the relationship between the development of cancer and the loss of function of a number of tumour suppressor genes. In the present study, we assessed methylation levels in the promoter region of SFN, S100A14 and POU2F3 genes in sarcoid samples to clarify whether DNA methylation may be associated with previously identified changes in the expression level of these genes during the course of tumour progression. Using bisulfite sequencing and clone sequencing, we detected that lesional samples had a significantly higher rate of DNA methylation in the analyzed S100A14A region than the corresponding normal skin tissue. A frequent methylation of the SFN and POU2F3 promoter sequences were observed in both the tumour samples and the control skin tissues. Further studies are needed to evaluate the role of aberrant methylation in sarcoid progression and to understand the mechanisms involved in reduced expression of SFN, S100A14 and POU2F3 genes in the lesional tissues.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > ISME Equine Clinic Bern > ISME Equine Clinic, Internal medicine 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) |
UniBE Contributor: |
Klukowska-Rötzler, Jolanta, Koch, Christoph |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0034-5288 |
Publisher: |
Association for Veterinary Teaching and Research Work |
Language: |
English |
Submitter: |
Andrea Gassmann-Suter |
Date Deposited: |
23 Jan 2019 11:37 |
Last Modified: |
05 Dec 2022 15:24 |
Publisher DOI: |
10.1016/j.rvsc.2018.07.006 |
Related URLs: |
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PubMed ID: |
30086514 |
Uncontrolled Keywords: |
DNA methylation Equine sarcoids POU2F3 S100A14 SFN Tumorigenesis |
BORIS DOI: |
10.7892/boris.123239 |
URI: |
https://boris.unibe.ch/id/eprint/123239 |
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