Seele, Jana; Tauber, Simone C; Bunkowski, Stephanie; Baums, Christoph G; Valentin-Weigand, Peter; de Buhr, Nicole; Beineke, Andreas; Iliev, Asparouh Iliev; Brück, Wolfgang; Nau, Roland (2018). The inflammatory response and neuronal injury in Streptococcus suis meningitis. BMC infectious diseases, 18(1), p. 297. BioMed Central 10.1186/s12879-018-3206-6
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BACKGROUND
Many of the currently used models of bacterial meningitis have limitations due to direct inoculation of pathogens into the cerebrospinal fluid or brain and a relatively insensitive assessment of long-term sequelae. The present study evaluates the utility of a Streptococcus (S.) suis intranasal infection model for the investigation of experimental therapies in meningitis.
METHODS
We examined the brains of 10 piglets with S. suis meningitis as well as 14 control piglets by histology, immunohistochemistry and in-situ tailing for morphological alterations in the hippocampal dentate gyrus and microglial activation in the neocortex.
RESULTS
In piglets with meningitis, the density of apoptotic neurons was significantly higher than in control piglets. Moreover, scoring of microglial morphology revealed a significant activation of these cells during meningitis. The slight increase in the density of dividing cells, young neurons and microglia observed in piglets suffering from meningitis was not statistically significant, probably because of the short time frame between onset of clinical signs and organ sampling.
CONCLUSIONS
The morphological changes found during S. suis meningitis are in accordance with abnormalities in other animal models and human autopsy cases. Therefore, the pig should be considered as a model for evaluating effects of experimental therapeutic approaches on neurological function in bacterial meningitis.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
UniBE Contributor: |
Iliev, Asparouh Iliev |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
1471-2334 |
Publisher: |
BioMed Central |
Language: |
English |
Submitter: |
Asparouh Iliev Iliev |
Date Deposited: |
12 Feb 2019 15:00 |
Last Modified: |
05 Dec 2022 15:24 |
Publisher DOI: |
10.1186/s12879-018-3206-6 |
PubMed ID: |
29970011 |
Uncontrolled Keywords: |
Immunohistochemistry Intranasal infection Meningitis Pig model Streptococcus suis |
BORIS DOI: |
10.7892/boris.123434 |
URI: |
https://boris.unibe.ch/id/eprint/123434 |
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