A Tomato Tocopherol-Binding Protein Sheds Light on Intracellular α-Tocopherol Metabolism in Plants.

Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; Romanò, Cecilia; Previtali, Viola; Almeida, Juliana; Bréhélin, Claire; Asis, Ramón; Quadrana, Leandro; Demarco, Diego; Alseekh, Saleh; Salinas Gamboa, Rigel; Pérez-Flores, Laura; Dominguez, Pia Guadalupe; Rothan, Christophe; Fernie, Alisdair Robert; González, Maurício; Stocker, Achim; ... (2018). A Tomato Tocopherol-Binding Protein Sheds Light on Intracellular α-Tocopherol Metabolism in Plants. Plant & cell physiology, 59(11), pp. 2188-2203. Oxford Academic 10.1093/pcp/pcy191

Text pcy191.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (1MB)

Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.

Interest & Impact

Downloads

3 since deposited on 19 Feb 2019
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item