A Tomato Tocopherol-Binding Protein Sheds Light on Intracellular α-Tocopherol Metabolism in Plants.

Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; Romanò, Cecilia; Previtali, Viola; Almeida, Juliana; Bréhélin, Claire; Asis, Ramón; Quadrana, Leandro; Demarco, Diego; Alseekh, Saleh; Salinas Gamboa, Rigel; Pérez-Flores, Laura; Dominguez, Pia Guadalupe; Rothan, Christophe; Fernie, Alisdair Robert; González, Maurício; Stocker, Achim; ... (2018). A Tomato Tocopherol-Binding Protein Sheds Light on Intracellular α-Tocopherol Metabolism in Plants. Plant & cell physiology, 59(11), pp. 2188-2203. Oxford Academic 10.1093/pcp/pcy191

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Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Stocker, Achim and Hemmerle, Andreas

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
500 Science > 580 Plants (Botany)

ISSN:

1471-9053

Publisher:

Oxford Academic

Language:

English

Submitter:

Andreas Hemmerle

Date Deposited:

19 Feb 2019 10:49

Last Modified:

19 Feb 2019 12:13

Publisher DOI:

10.1093/pcp/pcy191

PubMed ID:

30239816

BORIS DOI:

10.7892/boris.123547

URI:

https://boris.unibe.ch/id/eprint/123547

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