Computational prediction of inter-species relationships through omics data analysis and machine learning.

Leite, Diogo Manuel Carvalho; Brochet, Xavier; Resch, Grégory; Que, Yok-Ai; Neves, Aitana; Peña-Reyes, Carlos (2018). Computational prediction of inter-species relationships through omics data analysis and machine learning. BMC bioinformatics, 19(Suppl 14), p. 420. BioMed Central 10.1186/s12859-018-2388-7

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BACKGROUND Antibiotic resistance and its rapid dissemination around the world threaten the efficacy of currently-used medical treatments and call for novel, innovative approaches to manage multi-drug resistant infections. Phage therapy, i.e., the use of viruses (phages) to specifically infect and kill bacteria during their life cycle, is one of the most promising alternatives to antibiotics. It is based on the correct matching between a target pathogenic bacteria and the therapeutic phage. Nevertheless, correctly matching them is a major challenge. Currently, there is no systematic method to efficiently predict whether phage-bacterium interactions exist and these pairs must be empirically tested in laboratory. Herein, we present our approach for developing a computational model able to predict whether a given phage-bacterium pair can interact based on their genome. RESULTS Based on public data from GenBank and phagesDB.org, we collected more than a thousand positive phage-bacterium interactions with their complete genomes. In addition, we generated putative negative (i.e., non-interacting) pairs. We extracted, from the collected genomes, a set of informative features based on the distribution of predictive protein-protein interactions and on their primary structure (e.g. amino-acid frequency, molecular weight and chemical composition of each protein). With these features, we generated multiple candidate datasets to train our algorithms. On this base, we built predictive models exhibiting predictive performance of around 90% in terms of F1-score, sensitivity, specificity, and accuracy, obtained on the test set with 10-fold cross-validation. CONCLUSION These promising results reinforce the hypothesis that machine learning techniques may produce highly-predictive models accelerating the search of interacting phage-bacteria pairs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care

UniBE Contributor:

Que, Yok-Ai

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1471-2105

Publisher:

BioMed Central

Language:

English

Submitter:

Mirella Aeberhard

Date Deposited:

19 Feb 2019 08:13

Last Modified:

25 Oct 2019 07:42

Publisher DOI:

10.1186/s12859-018-2388-7

PubMed ID:

30453987

Uncontrolled Keywords:

Health Machine learning Phage-therapy Supervised learning

BORIS DOI:

10.7892/boris.123841

URI:

https://boris.unibe.ch/id/eprint/123841

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