ICAM-1 C57BL/6 Mice Are Not Protected from Experimental Ischemic Stroke.

Enzmann, Gaby; Pavlidou, Sofia; Vaas, Markus; Klohs, Jan; Engelhardt, Britta (2018). ICAM-1 C57BL/6 Mice Are Not Protected from Experimental Ischemic Stroke. Translational stroke research, 9(6), pp. 608-621. Springer 10.1007/s12975-018-0612-4

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Accumulation of neutrophils in the brain is a hallmark of cerebral ischemia and considered central in exacerbating tissue injury. Intercellular adhesion molecule (ICAM)-1 is upregulated on brain endothelial cells after ischemic stroke and considered pivotal in neutrophil recruitment as ICAM-1-deficient mouse lines were found protected from experimental stroke. Translation of therapeutic inhibition of ICAM-1 into the clinic however failed. This prompted us to investigate stroke pathogenesis in Icam1 C57BL/6 mutants, a true ICAM-1 mouse line. Performing transient middle cerebral artery occlusion, we found that absence of ICAM-1 did not ameliorate stroke pathology at acute time points after reperfusion. Near-infrared imaging showed comparable accumulation of neutrophils in the ischemic hemispheres of ICAM-1 and wild type C57BL/6 mice. We also isolated equal numbers of neutrophils from the ischemic brains of ICAM-1 and wild type C57BL/6 mice. Immunostaining of the brains showed neutrophils to equally accumulate in the leptomeninges and brain parenchymal vessels of ICAM-1 and wild type C57BL/6 mice. In addition, the lesion size was comparable in ICAM-1 and wild type mice. Our study demonstrates that absence of ICAM-1 neither inhibits cerebral ischemia-induced accumulation of neutrophils in the brain nor provides protection from ischemic stroke.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Enzmann, Gaby and Engelhardt, Britta

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1868-4483

Publisher:

Springer

Language:

English

Submitter:

Ursula Zingg-Zünd

Date Deposited:

25 Feb 2019 16:59

Last Modified:

18 Apr 2019 16:30

Publisher DOI:

10.1007/s12975-018-0612-4

PubMed ID:

29399739

Uncontrolled Keywords:

Blood-brain barrier Endothelium Focal ischemia Leukocytes Vascular biology

BORIS DOI:

10.7892/boris.124100

URI:

https://boris.unibe.ch/id/eprint/124100

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