A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models.

Lüönd, Fabiana; Bill, Ruben; Vettiger, Andrea; Oller, Heide; Pelczar, Pawel; Christofori, Gerhard (2019). A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models. Journal of mammary gland biology and neoplasia, 24(1), pp. 39-45. Springer 10.1007/s10911-018-9412-4

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Genetically engineered mouse models have become an indispensable tool for breast cancer research. Combination of multiple site-specific recombination systems such as Cre/loxP and Flippase (Flp)/Frt allows for engineering of sophisticated, multi-layered conditional mouse models. Here, we report the generation and characterization of a novel transgenic mouse line expressing a mouse codon-optimized Flp under the control of the mouse mammary tumor virus (MMTV) promoter. These mice show robust Flp-mediated recombination in luminal mammary gland and breast cancer cells but no Flp activity in non-mammary tissues, with the exception of limited activity in salivary glands. These mice provide a unique tool for studying mammary gland biology and carcinogenesis in mice.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology

UniBE Contributor:

Bill, Ruben

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1083-3021

Publisher:

Springer

Language:

English

Submitter:

Rebeka Gerber

Date Deposited:

08 Mar 2019 11:04

Last Modified:

19 Mar 2020 15:16

Publisher DOI:

10.1007/s10911-018-9412-4

PubMed ID:

30209717

Uncontrolled Keywords:

Breast cancer Flippase Lineage tracing MMTV promoter Mammary gland Transgenic mice

BORIS DOI:

10.7892/boris.124822

URI:

https://boris.unibe.ch/id/eprint/124822

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