Lüönd, Fabiana; Bill, Ruben; Vettiger, Andrea; Oller, Heide; Pelczar, Pawel; Christofori, Gerhard (2019). A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models. Journal of mammary gland biology and neoplasia, 24(1), pp. 39-45. Springer 10.1007/s10911-018-9412-4
|
Text
s10911-018-9412-4.pdf - Published Version Available under License Publisher holds Copyright. Download (5MB) | Preview |
Genetically engineered mouse models have become an indispensable tool for breast cancer research. Combination of multiple site-specific recombination systems such as Cre/loxP and Flippase (Flp)/Frt allows for engineering of sophisticated, multi-layered conditional mouse models. Here, we report the generation and characterization of a novel transgenic mouse line expressing a mouse codon-optimized Flp under the control of the mouse mammary tumor virus (MMTV) promoter. These mice show robust Flp-mediated recombination in luminal mammary gland and breast cancer cells but no Flp activity in non-mammary tissues, with the exception of limited activity in salivary glands. These mice provide a unique tool for studying mammary gland biology and carcinogenesis in mice.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology |
UniBE Contributor: |
Bill, Ruben |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1083-3021 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Rebeka Gerber |
Date Deposited: |
08 Mar 2019 11:04 |
Last Modified: |
05 Dec 2022 15:25 |
Publisher DOI: |
10.1007/s10911-018-9412-4 |
PubMed ID: |
30209717 |
Uncontrolled Keywords: |
Breast cancer Flippase Lineage tracing MMTV promoter Mammary gland Transgenic mice |
BORIS DOI: |
10.7892/boris.124822 |
URI: |
https://boris.unibe.ch/id/eprint/124822 |
Actions (login required)
 |
Edit item |