Klf4 transcription factor is expressed in the cytoplasm of prostate cancer cells

Le Magnen, Clémentine; Bubendorf, Lukas; Ruiz, Christian; Zlobec, Inti; Bachmann, Alexander; Heberer, Michael; Spagnoli, Giulio C.; Wyler, Stephen; Mengus, Chantal (2012). Klf4 transcription factor is expressed in the cytoplasm of prostate cancer cells. European journal of cancer, 49(4), pp. 955-663. Amsterdam: Elsevier 10.1016/j.ejca.2012.09.023

Full text not available from this repository.

BACKGROUND: Cancer initiation and progression might be driven by small populations of cells endowed with stem cell-like properties. Here we comparatively addressed the expression of genes encoding putative stemness regulators including c-Myc, Klf4, Nanog, Oct4A and Sox2 genes in benign prostatic hyperplasia (BPH) and prostate cancer (PCA). METHODS: Fifty-eight PCA and thirty-nine BPH tissues samples were used for gene expression analysis, as evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The expression of specific Klf4 isoforms was tested by conventional PCR. Klf4 specific antibodies were used for protein detection in a tissue microarray including 404 prostate samples. RESULTS: Nanog, Oct4A and Sox2 genes were comparably expressed in BPH and PCA samples, whereas c-Myc and Klf4 genes were expressed to significantly higher extents in PCA than in BPH specimens. Immunohistochemical studies revealed that Klf4 protein is detectable in a large majority of epithelial prostatic cells, irrespective of malignant transformation. However, in PCA, a predominantly cytoplasmic location was observed, consistent with the expression of a differentially spliced Klf4α isoform. CONCLUSION: Klf4 is highly expressed at gene and protein level in BPH and PCA tissues but a cytoplasmic location of the specific gene product is predominantly detectable in malignant cells. Klf4 location might be of critical relevance to steer its functions during oncogenesis.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute of Pathology
UniBE Contributor:	Zlobec, Inti
ISSN:	0959-8049
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:32
Last Modified:	05 Dec 2022 14:10
Publisher DOI:	10.1016/j.ejca.2012.09.023
PubMed ID:	23089465
Web of Science ID:	000314849300024
URI:	https://boris.unibe.ch/id/eprint/12493 (FactScience: 218845)