DOPS Adjuvant Confers Enhanced Protection against Malaria for VLP-TRAP Based Vaccines.

Cabral de Miranda, Gustavo; M Salman, Ahmed; Mohsen, Mona Omar Mahmoud; Storni, Federico Lorenzo; Rösti, Elisa Simona; A Skinner, Murray; D Heath, Matthew; F Kramer, Matthias; M Khan, Shahid; J Janse, Chris; V S Hill, Adrian; Bachmann, Martin (2018). DOPS Adjuvant Confers Enhanced Protection against Malaria for VLP-TRAP Based Vaccines. Diseases, 6(4) MDPI 10.3390/diseases6040107

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Vaccination remains the most effective and essential prophylactic tool against infectious diseases. Enormous efforts have been made to develop effective vaccines against malaria but successes remain so far limited. Novel adjuvants may offer a significant advantage in the development of malaria vaccines, in particular if combined with inherently immunogenic platforms, such as virus-like particles (VLP). Dioleoyl phosphatidylserine (DOPS), which is expressed on the outer surface of apoptotic cells, represents a novel adjuvant candidate that may confer significant advantage over existing adjuvants, such as alum. In the current study we assessed the potential of DOPS to serve as an adjuvant in the development of a vaccine against malaria either alone or combined with VLP using thrombospondin-related adhesive protein (TRAP) as a target antigen. TRAP was chemically coupled to VLPs derived from the cucumber mosaic virus fused to a universal T cell epitope of tetanus toxin (CuMVtt). Mice were immunized with TRAP alone or formulated in alum or DOPS and compared to TRAP coupled to CuMVtt formulated in PBS or DOPS. Induced immune responses, in particular T cell responses, were assessed as the major protective effector cell population induced by TRAP. The protective capacity of the various formulations was assessed using a transgenic expressing PfTRAP. All vaccine formulations using adjuvants and/or VLP increased humoral and T cell immunogenicity for PfTRAP compared to the antigen alone. Display on VLPs, in particular if formulated with DOPS, induced the strongest and most protective immune response. Thus, the combination of VLP with DOPS may harness properties of both immunogenic components and optimally enhance induction of protective immune responses.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology (discontinued)

UniBE Contributor:

Cabral de Miranda, Gustavo; Mohsen, Mona Omar Mahmoud; Storni, Federico Lorenzo; Rösti, Elisa Simona and Bachmann, Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2079-9721

Publisher:

MDPI

Language:

English

Submitter:

Valery Beer

Date Deposited:

01 Apr 2019 12:29

Last Modified:

20 Jun 2019 11:39

Publisher DOI:

10.3390/diseases6040107

PubMed ID:

30469323

Uncontrolled Keywords:

Plasmodium falciparum adjuvants dioleoyl phosphatidylserine (DOPS) malaria vaccine virus like particle (VLP)

BORIS DOI:

10.7892/boris.125730

URI:

https://boris.unibe.ch/id/eprint/125730

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