In vitro-microenvironment directs preconditioning of human chorion derived MSC promoting differentiation of OPC-like cells.

Periasamy, Ramesh; Surbek, Daniel; Schoeberlein, Andreina (2018). In vitro-microenvironment directs preconditioning of human chorion derived MSC promoting differentiation of OPC-like cells. Tissue and cell, 52, pp. 65-70. Elsevier 10.1016/j.tice.2018.04.002

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The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown either in serum replacement medium (SRM) or serum-containing medium (SM) and tested for their morphology, proliferation, secretome, migration, protein expression and differentiation into OPC-like cells. MSC cultured in SRM condition have distinct morphology/protein expression profile, increased cell proliferation/migration and capacity to differentiate into OPC-like cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin

UniBE Contributor:

Surbek, Daniel and Schoeberlein, Andreina

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0040-8166

Publisher:

Elsevier

Language:

English

Submitter:

Monika Zehr

Date Deposited:

01 Apr 2019 12:55

Last Modified:

24 Oct 2019 08:24

Publisher DOI:

10.1016/j.tice.2018.04.002

PubMed ID:

29857830

Uncontrolled Keywords:

Chorion; Culture conditions; Extracellular matrix; Mesenchymal stem cells; Microenvironment; Neurogenic differentiation; OPC; Placenta; Stem cells

BORIS DOI:

10.7892/boris.125736

URI:

https://boris.unibe.ch/id/eprint/125736

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