PCR Diagnosis of Small Hive Beetles

Ouessou Idrissou, Franck; Huang, Qiang; Yanez Amayo, Victor Orlando; Akinwande, Kayode; Neumann, Peter (2018). PCR Diagnosis of Small Hive Beetles. Insects, 9(1) MDPI 10.3390/insects9010024

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Small hive beetles (SHBs), Aethinatumida, are parasites of social bee colonies native to sub-Saharan Africa and have become an invasive species at a global scale. Reliable Polymerase Chain Reaction (PCR) diagnosis of this mandatory pest is required to limit its further spread and impact. Here, we have developed SHB primers, which amplify for 10 native African locations and 10 reported introductions, but not for three closely related species (Aethinaconcolor, Aethinaflavicollis, and Aethinainconspicua). We also show that adult honey bee workers can be used as matrices for PCR-based detection of SHBs. The sensitivity of this novel method appears to be 100%, which is identical to conventional visual screenings. Furthermore, the specificity of this novel approach was also high (90.91%). Since both sensitivity and specificity are high, we recommend this novel PCR method and the new primers for routine surveillance of hives in high-risk areas.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Bee Health
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Ouessou Idrissou, Franck; Huang, Qiang; Yanez Amayo, Victor Orlando and Neumann, Peter

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

2075-4450

Publisher:

MDPI

Language:

English

Submitter:

Anna Papach

Date Deposited:

21 May 2019 14:37

Last Modified:

24 Oct 2019 02:07

Publisher DOI:

10.3390/insects9010024

PubMed ID:

29443937

BORIS DOI:

10.7892/boris.127022

URI:

https://boris.unibe.ch/id/eprint/127022

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