Developing Bioreactors to Host Joint-Derived Tissues That Require Mechanical Stimulation

Gantenbein, Benjamin; Frauchiger, Daniela Angelika; May, Rahel; Bakirci, Ezgi; Rohrer, Urs; Grad, Sibylle (2019). Developing Bioreactors to Host Joint-Derived Tissues That Require Mechanical Stimulation. In: Reference Module in Biomedical Sciences. Elsevier 10.1016/B978-0-12-801238-3.65611-8

[img] Text
Developing Bioreactors to Host Joint-Derived Tissues That Require Mechanical Stimulation.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (2MB) | Request a copy

Demographics of the Western Societies points toward an elderly population in need of research on replacement parts for joints and their components, such as the meniscus, cartilage, ligaments, tendons, and intervertebral discs. There is a lack of basic research to predict treatment options before degeneration or inflammation has progressed, and at late stages, when regeneration might not be an option anymore. Thus, to achieve a better understanding of the current specific problems in orthopedic research, there is a need for clinically relevant mechanobiological models. Animal experiments, especially those on large animals, are costly and, in some cases, doubtful as regards clinical translation. Ex vivo bioreactors that allow biomechanical loading are aimed to mimic the in vivo situation of critical joints that are prone to failure. These tissues often require unique adaptations prior and during organ culture as these are often under mechanical forces in situ. On the one hand, ex vivo organ cultures are limited in regarding the size and cell numbers that can be kept alive and the duration of experiments. However, a strong asset of these cultures is the use of primary human material, which is a chance to provide more translational relevant results. Within this book chapter, we give a brief history of general concepts for bioreactor constructions in the field of orthopedic research and give some recent examples for tendons, the knee joint and the intervertebral disc. We offer a summary of the current state of the art, pitfalls and limitations in the design and the future challenges.

Item Type:

Book Section (Encyclopedia Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Gantenbein, Benjamin; Frauchiger, Daniela Angelika; May, Rahel; Bakirci, Ezgi and Rohrer, Urs

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISBN:

978-0-12-801238-3

Publisher:

Elsevier

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

20 May 2019 18:28

Last Modified:

22 Oct 2019 20:53

Publisher DOI:

10.1016/B978-0-12-801238-3.65611-8

Uncontrolled Keywords:

Anterior cruciate ligament, Bioreactor, Cartilage, Cell viability, Extracellular matrix, Force-controlled loading, Gene expression, Intervertebral disc, Mechanical loading, Organ culture, Read-out parameters, Regenerative therapy, Strain-controlled loading, Tendon

BORIS DOI:

10.7892/boris.128465

URI:

https://boris.unibe.ch/id/eprint/128465

Actions (login required)

Edit item Edit item
Provide Feedback