Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects.

Surowska, Anna; Jegatheesan, Prasanthi; Campos, Vanessa; Marques, Anne-Sophie; Egli, Léonie; Cros, Jérémy; Rosset, Robin; Lecoultre, Virgile; Kreis, Roland; Boesch, Christoph Hans; Pouymayou, Bertrand Michel Marie; Kreis, Roland; Tappy, Luc (2019). Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects. Nutrients, 11(1) Molecular Diversity Preservation International MDPI 10.3390/nu11010209

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Sucrose overfeeding increases intrahepatocellular (IHCL) and intramyocellular (IMCL) lipid concentrations in healthy subjects. We hypothesized that these effects would be modulated by diet protein/fat content. Twelve healthy men and women were studied on two occasions in a randomized, cross-over trial. On each occasion, they received a 3-day 12% protein weight maintenance diet (WM) followed by a 6-day hypercaloric high sucrose diet (150% energy requirements). On one occasion the hypercaloric diet contained 5% protein and 25% fat (low protein-high fat, LP-HF), on the other occasion it contained 20% protein and 10% fat (high protein-low fat, HP-LF). IHCL and IMCL concentrations (magnetic resonance spectroscopy) and energy expenditure (indirect calorimetry) were measured after WM, and again after HP-LF/LP-HF. IHCL increased from 25.0 ± 3.6 after WM to 147.1 ± 26.9 mmol/kg wet weight (ww) after LP-HF and from 30.3 ± 7.7 to 57.8 ± 14.8 after HP-LF (two-way ANOVA with interaction: p < 0.001 overfeeding x protein/fat content). IMCL increased from 7.1 ± 0.6 to 8.8 ± 0.7 mmol/kg ww after LP-HF and from 6.2 ± 0.6 to 6.9 ± 0.6 after HP-LF, (p < 0.002). These results indicate that liver and muscle fat deposition is enhanced when sucrose overfeeding is associated with a low protein, high fat diet compared to a high protein, low fat diet.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Abt. Magnetresonanz-Spektroskopie und Methodologie, AMSM

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Boesch, Christoph Hans; Pouymayou, Bertrand Michel Marie and Kreis, Roland

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2072-6643

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Maria de Fatima Henriques Bernardo

Date Deposited:

20 May 2019 18:32

Last Modified:

24 Oct 2019 07:39

Publisher DOI:

10.3390/nu11010209

PubMed ID:

30669704

Uncontrolled Keywords:

dietary fat content dietary protein content energy expenditure hepatic steatosis intrahepatocellular lipids intramyocellular lipids plasma triglyceride sucrose overfeeding

BORIS DOI:

10.7892/boris.128507

URI:

https://boris.unibe.ch/id/eprint/128507

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